Dopamine regulates endothelial progenitor cell mobilization from mouse bone marrow in tumor vascularization
J. Clin. Invest. Debanjan Chakroborty, et al. 118:1380 doi:10.1172/JCI33125 [Go to this article.]

Figure 1
Transplantation of S180 tumors in normal mice significantly increases CEPC numbers and decreases DA concentration in the BM. (A and B) Corresponding isotype controls. (C–G) Enumeration of CEPC numbers in normal and tumor-bearing mice. Significantly increased numbers of CEPCs were detected in tumor-bearing mice but were undetectable in the blood samples of normal mice. Cells were initially gated to exclude dead cells, debris, and red blood cells. (C and E) Subsequent gate used to select total CD45^– cell population. (D and F) Corresponding flow cytometric analysis for detecting CD34⁺VEGFR2⁺ cells in the gated CD45^– cell population. The cells represented in the upper-right quadrant are the desired EPC population showing a phenotype of CD45^–CD34⁺VEGFR2⁺ cells. (G) Absolute number of EPCs (*P < 0.05). Figures represent EPC frequency on posttransplantation day 6 and are representative of 4 separate experiments for each group. (H) BM DA concentration was found to decrease significantly from day 3 after tumor transplant, showing an inverse correlation with mobilization of EPCs.