Impact of bacteria on the phenotype, functions, and therapeutic activities of invariant NKT cells in mice
J. Clin. Invest. Sungjune Kim, et al. 118:2301 doi:10.1172/JCI33071 [Go to this article.]

Figure 2
The in vitro response of splenocytes to α-GalCer from mice treated 3 weeks earlier with α-GalCer or bacteria. Mice were injected with α-GalCer (5 μg/mouse, i.p.) or the indicated bacteria and sacrificed 3 weeks later, and splenocytes (2 × 10⁵ per well) were cultured with graded doses of α-GalCer. After 3 days, proliferation was assessed by [³H]thymidine incorporation, and culture supernatants were evaluated for IL-4 and IFN-γ levels by ELISA. Proliferation and cytokine results represent the mean ± SEM of 6–11 mice per group, pooled from 3 experiments. *P < 0.05 compared with naive splenocytes cultured with the same dose of α-GalCer.