TRAIL-R deficiency in mice enhances lymph node metastasis without affecting primary tumor development
J. Clin. Invest. Anne Grosse-Wilde, et al. 118:100 doi:10.1172/JCI33061 [
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Figure 5Detachment-induced inactivation of ERK sensitizes to TRAIL- and CD95L-induced apoptosis. (
A) Cells were incubated attached or detached for 12 hours. Cell surface–expressed TRAIL-R and CD95 were detected on the indicated skin carcinoma cell lines. Representative flow cytometry profiles of at least 2 independent experiments are shown. (
B) DT02 cells and (
C) DT01 cells were trypsinized (0 h) and incubated either detached or attached for the indicated times. Western blots were incubated with the indicated antibodies. In
B, note that c-FLIP
L, but not c-FLIP
S, was detected. (
D) DT02 cells were trypsinized and seeded into noncoated 24-well plates either with or without U0126 (40 μM) and TRAIL or CD95L at the indicated concentrations for 24 hours. Cell death was determined as described in Figure
4. Standard deviation for duplicates is shown. Results are representative of 3 independent experiments.
