Human and simian immunodeficiency viruses deregulate early hematopoiesis through a Nef/PPARγ/STAT5 signaling pathway in macaques
J. Clin. Invest. Stéphane Prost, et al. 118:1765 doi:10.1172/JCI33037 [Go to this article.]

Figure 1
SIV inhibits the clonogenic potential of hematopoietic progenitors through downregulation of STAT5. (A) Evaluation of total progenitor cell counts in semisolid cultures of CD34⁺ BM cells collected from 5 animals before and after infection with SIV. Day 0 is the day of SIV injection. Horizontal lines indicate mean CFC numbers scored from all cell cultures from the 5 animals analyzed at the indicated time. Each symbol represents samples from a single animal. (B) STAT5B mRNA was evaluated by RT-PCR in CD34⁺ BM cells at various times following animal infection with SIV. Levels were normalized to GAPDH mRNA. Results were expressed relative to the average level of STAT5B mRNA in samples collected before SIV injection. Horizontal lines indicate the mean values for STAT5B mRNA in 5 animal samples at the indicated time. STAT5A mRNA was similarly evaluated and compared in noninfected control (NI) and chronically SIV-infected animals (SIV) (inset). (C) CD34⁺ BM cells from 3 noninfected control and 3 SIVmac251-infected macaques at 93 days after injection were lysed. Lysates were subjected to SDS-PAGE and western blotting. STAT5 and actin proteins were detected by coincubation with pan STAT5 and anti-actin antibodies. (D) CD34⁺ BM cells from 4 control or 4 chronically SIV-infected macaques were transduced with or without a lentiviral vector encoding simian STAT5B. They were processed for CFC assays. Experiments were repeated twice. Horizontal lines indicate the mean number of colonies scored in each condition. Each symbol represents samples from a single animal.