Leptin inhibits 4-aminopyridine– and pentylenetetrazole-induced seizures and AMPAR-mediated synaptic transmission in rodents
J. Clin. Invest. Lin Xu, et al. 118:272 doi:10.1172/JCI33009 [Go to this article.]

Figure 6
Leptin inhibits low magnesium–induced spiking in cultured mouse hippocampal neurons. (A) Whole-cell patch recordings from cultured hippocampal neurons in current clamp configuration spontaneously fire action potentials in nominally magnesium-free external solution. Baseline membrane potential: –57 mV. Calibration for A1–A3: 10 s, 30 mV. (B) Leptin (1 nM) reduces action potential firing frequency by 30%–40%. (C) Action potential firing recovers close to baseline frequency after leptin washout. (D) Cumulative data comparing spike frequency in low magnesium before (filled squares) and after (open squares) application of different leptin concentrations. Each filled and open symbol connected by a line represents 1 cell and its firing frequency before and after leptin. Therefore, each line represents 1 matched data pair; some data points are superimposed on each other. The mean ± SD values are presented beside each data set with a larger symbol. A significant reduction in spike frequency was produced by 1.0 nM (n = 13 cells) and 10 nM (n = 11 cells), but not by 0.1 nM (n = 12 cells) leptin (paired t test).