Leptin inhibits 4-aminopyridine– and pentylenetetrazole-induced seizures and AMPAR-mediated synaptic transmission in rodents
J. Clin. Invest. Lin Xu, et al. 118:272 doi:10.1172/JCI33009 [Go to this article.]

Figure 5
Leptin inhibits AMPAR-mediated EPSCs obtained from voltage-clamped CA1 pyramidal neurons in mouse hippocampal slices. (A) Representative tracings of Schaffer collateral evoked whole-cell EPSCs recorded from a voltage-clamped CA1 neuron at –70 mV at baseline during perfusion of ACSF containing 2 mM CaCl₂ and 1 mM MgCl₂ (Baseline), after a 10-minute perfusion with 0.6 nM leptin (Leptin), and after a 20-minute washout with ACSF (Recovery). Calibration: 5 ms, 25 pA. (B) Leptin inhibited AMPAR-mediated EPSCs with a U-shaped dose-response relation. Peak amplitudes of AMPAR-mediated EPSCs after a 10-minute perfusion with various leptin concentrations expressed as a percentage of the peak amplitude of the baseline EPSC (n = 5–9 cells). *P < 0.001 for 0.6 vs. 0.06 nM and *P < 0.01 for 0.6 vs. 50 nM leptin (ANOVA followed by Tukey-Kramer’s test for multiple comparisons). (C) Leptin-enhanced paired pulse facilitation. The paired-pulse ratio was calculated as in Figure 4 by using peak amplitudes of pairs of EPSCs evoked with an interstimulus interval of 25 milliseconds in 0.6 nM leptin. n = 8 cells, *P < 0.02 (paired t test).