Leptin inhibits 4-aminopyridine– and pentylenetetrazole-induced seizures and AMPAR-mediated synaptic transmission in rodents
J. Clin. Invest. Lin Xu, et al. 118:272 doi:10.1172/JCI33009 [Go to this article.]

Figure 3
Leptin inhibits CA1 fEPSPs in mouse hippocampal slices. (A) Representative tracings of Schaffer collateral evoked fEPSPs recorded in CA1 at baseline during perfusion of ACSF (Baseline), after a 20-minute perfusion with 0.6 nM leptin (Leptin), and after a 30-minute washout with ACSF (Recovery). Calibration: 2 ms, 0.1 mV. (B) Time course of the fEPSP slope demonstrates the inhibition of fEPSP during a 20-minute application of 0.6 nM leptin (indicated by the bar), which almost completely recovers after a 30-minute washout with ACSF (n = 7 slices, 6 mice). fEPSP slopes at each time point were normalized to the slope of the baseline fEPSPs, which is indicated by the dotted line. Only every third point is shown for clarity. (C) U-shaped concentration-response curve of leptin inhibition of the fEPSP slope demonstrates maximal inhibition at 0.6 nM and minimal inhibition at 0.006 and 50 nM leptin (n = 6 slices, 2 mice).