Renin inhibition reduces hypercholesterolemia-induced atherosclerosis in mice
J. Clin. Invest. Hong Lu, et al. 118:984 doi:10.1172/JCI32970 [Go to this article.]

Figure 7
Renin deficiency in mouse macrophages markedly reduced monocyte adhesion to endothelial cells. (A) AngII resulted in increased THP-1 monocyte adhesion to endothelial cells. The adhesion assay was performed by coincubation of 10^–7 M AngII with HUVECs for 18 hours. Values (mean ± SEM) represent cell number of each well relative to vehicle per HPF (n = 4 per group). *P = 0.009 versus vehicle. (B) The AT1 receptor antagonist losartan inhibited AngII-induced THP-1 monocyte adhesion to endothelial cells. The adhesion assay was performed by coincubation of 10^–7 M AngII and 10^–6 M losartan. Losartan was added 2 hours prior to the addition of AngII to cultured HUVECs. Values (mean ± SEM) represent cell number of each well relative to losartan per HPF (n = 3 per group). *P < 0.001 versus AngII. (C) Renin deficiency resulted in decreased THP-1 monocyte adhesion to HUVECs. Cultured peritoneal macrophages from renin^+/+ or renin^–/– mice were coincubated with HUVECs for 24 hours, and an adhesion assay was then performed. Values (mean ± SEM) represent cell number of each well relative to renin^–/– per HPF (n = 4 per group). *P = 0.03 versus renin^+/+.