Renin inhibition reduces hypercholesterolemia-induced atherosclerosis in mice
J. Clin. Invest. Hong Lu, et al. 118:984 doi:10.1172/JCI32970 [
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Figure 6AT1a receptor deficiency in bone marrow–derived cells did not alter atherosclerotic lesion size. (
A) RT-PCR confirmed that macrophages expressed mRNA of AT1a receptors (AT1aR), but not of AT1b receptors. Examples of positive controls (adrenal glands and aorta) and macrophages for AT1a receptors, AT1b receptors, and 18S rRNA. Lane 1, adrenal gland; lane 2, aorta; lane 3, macrophages; lane 4, non-RT negative control in adrenal glands. The lanes were run on the same gel but were noncontiguous. (
B) PCR confirmed the presence of the disrupted AT1a receptor gene in mouse peritoneal macrophages from recipient mice. PCR on macrophage-derived DNA yielded an amplicon of 650 bp for wild-type AT1a receptor and 1.1 kb for the disrupted allele. (
C) Atherosclerotic lesion size was measured on aortic arch intimal surfaces. Individual symbols represent Ldlr
–/– recipients. Means of each group are represented by circles; error bars denote SEM.
n = 25 (AT1a receptor
+/+ donor); 24 (AT1a receptor
–/– donor).
