Acute effects of leptin require PI3K signaling in hypothalamic proopiomelanocortin neurons in mice
J. Clin. Invest. Jennifer W. Hill, et al. 118:1796 doi:10.1172/JCI32964 [
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Figure 4Deletion of exon 7 of
Pik3r1 in genomic DNA from hypothalamic nuclei of
Pik3r1 POMCKO Pik3r2–/– mice.
(
A) The 1,275-bp region between primer binding sites becomes 298 bp in length when exon 7 is excised by cre recombinase. (
B) Amplified genomic DNA from a
Pik3r1 POMCKO Pik3r2–/– and littermate control (Con) mice. Tail DNA from a
Pik3r1–/– mouse is shown as a positive control (+). Olf, olfactory bulb DNA; HYP, DNA from whole hypothalamus; DMH, dorsomedial hypothalamic nucleus; PVN, paraventricular nucleus.
