Acute effects of leptin require PI3K signaling in hypothalamic proopiomelanocortin neurons in mice
J. Clin. Invest. Jennifer W. Hill, et al. 118:1796 doi:10.1172/JCI32964 [Go to this article.]

Figure 1
Leptin increases POMC neuronal activity in a PI3K-dependent manner. (A) Sample traces of loose-patch recording in control ACSF, with leptin (100 nM), after wash to control ACSF, and in the presence of the PI3K inhibitor wortmannin (100 nM). (B) Histogram showing spike frequency of the same neuron in A organized into 20-s epochs. (C) Leptin-induced increase in neuronal activity of identified POMC neurons. P values compared with control ACSF are shown.