Recovery from diabetes in mice by β cell regeneration
J. Clin. Invest. Tomer Nir, et al. 117:2553 doi:10.1172/JCI32959 [Go to this article.]

Figure 3
Cell proliferation during regeneration. (A) Proliferation of differentiated (insulin⁺) β cells in 5-week-old mice treated with doxycycline between 4 and 5 weeks of age. Arrowheads denote proliferating β cells. (B) Proliferation of β cells following cell ablation and regeneration. Three days after the addition of doxycycline to the drinking water of 4-week-old mice, while fed and fasting glucose levels as well as glucose tolerance were still normal (not shown), the rate of β cell proliferation increased about 2.5-fold. The high rate of β cell proliferation was also seen after 7 days of doxycycline treatment and persisted 2 weeks after doxycycline withdrawal (i.e., 7-week-old mice, treated between weeks 4 and 5). (C) Exocrine cell proliferation during β cell ablation and regeneration. At the peak of β cell ablation (i.e., in 5-week-old mice, treated with doxycycline between weeks 4 and 5), a transient increase in exocrine cell proliferation was observed, possibly the result of an inflammatory process. Values are mean ± SD from 3 mice per time point. More than 1,000 β cells and 5,000 exocrine cells were counted per mouse. **P < 0.01.