Curcumin prevents and reverses murine cardiac hypertrophy
J. Clin. Invest. Hong-Liang Li, et al. 118:879 doi:10.1172/JCI32865 [
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Figure 5Pretreatment with curcumin blocks GATA4 activation. (
A and
B) Curcumin blocked the acetylation and DNA-binding activity of GATA4 induced by PE infusion (
A) or AB (
B).
n = 4. Oct-1 DNA-binding activity was used as a control. (
C) Effect of p300 on the acetylation and DNA-binding activity of GATA4 induced by PE. Cells were infected with Ad-p300, Ad-DN-p300, or Ad-GFP for 24 hours and then treated with 100 μM PE for 24 hours. Extracts were assayed for GATA4 acetylation and DNA-binding activity. (
D) p300 partially reversed the inhibitory effect of curcumin on the acetylation and DNA-binding activity of GATA4 induced by PE. Cells were infected with Ad-p300 or Ad-GFP for 24 hours, treated with 25 μM curcumin for 60 minutes, and then incubated with 100 μM PE for 24 hours. The results were reproducible in 3 separate experiments.
