Transplanted endothelial cells repopulate the liver endothelium and correct the phenotype of hemophilia A mice
J. Clin. Invest. Antonia Follenzi, et al. 118:935 doi:10.1172/JCI32748 [Go to this article.]

Figure 3
Integrity of transplanted LSECs in the liver. (A) TEM after GFP immunostaining showing integration of transplanted LSECs after 1 week (arrows) with characteristic layering of hepatic sinusoidal endothelium (arrowheads). Asterisk indicates lipid-laden stellate cells in adjacent area. (B–F) Analysis of DiI-Ac-LDL uptake (red) in GFP-positive LSECs (green) producing yellow color in cells containing both. Shown are transplanted GFP-positive LSECs in FVB/N mice containing green, yellow, or red domains due to DiI-Ac-LDL uptake in cells 3 months (E) after cell transplantation. (G–J) Combined staining for GFP (H) and CD31 (I) verifying transplanted cells expressed both markers (yellow). (K–N) Costaining for F4/80 (L, red) and GFP (M, green) showing absence of F4/80 immunostaining in transplanted LSECs, indicating distinction from Kupffer cells. (G and K) Nuclei stained with DAPI (blue). Original magnification, ×2,700 (A); ×200 (B); ×400 (C–F); ×630 (G–N). Scale bars: 5 μm (A); 10 μm (G–N).