Secretoneurin promotes neuroprotection and neuronal plasticity via the Jak2/Stat3 pathway in murine models of stroke
J. Clin. Invest. Woei-Cherng Shyu, et al. 118:133 doi:10.1172/JCI32723 [Go to this article.]

Figure 4
SN administration i.v. 30 minutes after cerebral ischemia rescued neurons from ischemic injury by directly inhibiting apoptotic signals. (A and B) Representative triphenyltetrazolium chloride–stained brain slices from rats injected with SN and vehicle. (C–N) Sections from the brain slices in A and B were immunostained to identify MAP-2⁺ neurons (C–H) and Neu-N⁺ neurons (I–N) in 3 different regions (corresponding with symbols in A and B). (O) The number of MAP-2– and Neu-N–immunoreactive cells in the penumbral area (shown in D, G, J, and M) was significantly increased in SN-treated rats compared with control rats. (P and Q) Significantly increased expression of Bcl-2 and Bcl-xL in SN-treated rats. (R) Increased expression of p-Stat3 in SN-treated rats, which was knocked down by AG490. Data are mean ± SEM. *P < 0.05 vs. control. Scale bar: 40 μm.