Regulation and roles of PKR in the interaction of CS and poly(I:C).
Mice were exposed to CS or RA (CS–) for 2 weeks and then given 4 doses of poly(I:C) (50 μg) or vehicle control. (A) Effects of poly(I:C) on PKR phosphorylation. The roles of IL-18Rα and IFN-γ in this activation are seen in comparisons of the levels of PKR phosphorylation in WT (+/+) mice and mice with null (–/–) mutations of IL-18Rα or IFN-γ (B). The role or roles of MAVS in this activation are seen in comparisons of the levels of PKR phosphorylation in WT (+/+) mice and mice with null (–/–) mutations of MAVS (C). The roles of PKR in poly(I:C)-induced total (D) and differential (E) BAL cell recovery and emphysema (F) are also illustrated. The values in parts D–F represent the mean ± SEM of evaluations in a minimum of 5 mice. The data depicted in each row in parts A–C were generated from blots of individual gels that were run at the same time. Parts A–C are representative of a minimum of 3 similar evaluations. *P < 0.05; **P < 0.01.