Csf3r mutations in mice confer a strong clonal HSC advantage via activation of Stat5
J. Clin. Invest. Fulu Liu, et al. 118:946 doi:10.1172/JCI32704 [Go to this article.]

Figure 7
Competitive repopulation by HSC expressing the d715F G-CSFR is impaired. (A and B) Wild-type or d715F G-CSFR KSL cells were treated in vitro for the indicated times with G-CSF (100 ng/ml) and then incubated with antibodies specific for pStat5 (A) or pStat3 (B). The MFI of triplicate experiments is shown. (C–E) Irradiated syngeneic mice were reconstituted with a 1:1 ratio of d715F G-CSFR and wild-type bone marrow cells. The resulting chimeric mice were treated 4 months after bone marrow transplantation with saline alone or G-CSF (10 μg/kg/d) for 21 days. The percentage of circulating neutrophils (C), B lymphocytes (D), and T lymphocytes (E) derived from d715F G-CSFR cells was determined at the indicated times (n = 3–4 for each group). Data represent the mean ± SEM. *P < 0.05 compared with saline-treated mice.