Csf3r mutations in mice confer a strong clonal HSC advantage via activation of Stat5
J. Clin. Invest. Fulu Liu, et al. 118:946 doi:10.1172/JCI32704 [
Go to this article.]
Figure 5Stat5 but not Stat3 activation by G-CSF is enhanced in d715 G-CSFR cells. Wild-type or d715 G-CSFR bone marrow cells were treated in vitro for the indicated times with G-CSF (100 ng/ml), and the cells were then incubated with antibodies specific for phosphorylated Stat5 or Stat3. (
A and
B) The MFI of Gr-1
+ (myeloid) cells for pStat5 (
A) and pStat3 (
B) is shown. (
C and
D) The MFI of KSL cells for pStat5 (
C) and pStat3 (
D) is shown. Data represent the mean ± SEM (
n = 4 per cohort). *
P < 0.05 compared with wild-type cells at the same time point.
