Csf3r mutations in mice confer a strong clonal HSC advantage via activation of Stat5
J. Clin. Invest. Fulu Liu, et al. 118:946 doi:10.1172/JCI32704 [Go to this article.]

Figure 4
G-CSF–induced proliferation of d715 G-CSFR HSCs is increased. Wild-type or d715 G-CSFR mice were treated with saline or G-CSF (2 doses of 100 μg/kg, 12 hours apart); in some cases (C and D), mice also received BrdU (2 doses of 1 mg, 12 hours apart). (A) Representative histograms showing Hoechst staining of KSL cells. (B). Shown is the percentage of KSL cells in the S/G₂/M phases of the cell cycle (n = 3–4 per condition). (C) Representative scatter plots showing the gating strategy and BrdU staining of CD150⁺CD48^–CD41^–lineage^– cells. (D) Shown is the percentage of CD150⁺CD48^–CD41^–lineage^– cells that labeled with BrdU (n = 5–6 per condition). Data represent the mean ± SEM.