A CD28 superagonistic antibody elicits 2 functionally distinct waves of T cell activation in rats
J. Clin. Invest. Nora Müller, et al. 118:1405 doi:10.1172/JCI32698 [Go to this article.]

Figure 5
JJ316 strongly impacts T cell adhesion and locomotion on fibronectin ex vivo. (A) CD4⁺ lymph node T cells were purified before and 12 hours after administration of 1.0 mg JJ316, added onto fibronectin-coated surfaces, and imaged ex vivo by confocal microscopy over a 10-minute time period. Scale bar: 5 μm. The migration path of 25 randomly chosen cells is shown for the tracking analysis of imaged time stacks. The graph is representative of 3 independent experiments. (B) CD4⁺CD25^– Th and CD4⁺CD25⁺ Tregs were purified on the basis of CD25-surface expression 72 hours after injection of JJ316, added on fibronectin-coated slides, and imaged by confocal microscopy over a 10 minutes time period (only the first 100 seconds are displayed in the photographic series). Scale bar: 5 mm. The migration path of 25 randomly chosen cells is shown for the tracking analysis. The graph is representative of 3 independent experiments.