Figure 9
Involvement of CXCR4 in thrombus formation.
Wild-type mice were infused with Alexa Fluor 647–conjugated anti-mouse CD41 Fab fragment (0.25 μg/g mouse), pAbantipeptide (0.6 μg/g mouse), and Alexa Fluor 488–conjugated goat anti-rabbit antibody (0.6 μg/g mouse) prior to injury. (A) Top row: Thrombus formation after laser injury in the absence of AMD3100. Bottom row: Thrombus formation after laser injury following infusion of AMD3100. Fluorescence signal of accumulated anti-CD41 antibody, red; fluorescence signal of accumulated pAbantipeptide, green; merge, yellow. Original magnification, ×600. (B) Median BSDL (pAbantipeptide) integrated fluorescence intensity in laser-induced thrombi in wild-type mice before and after treatment as in A with AMD3100 (1.25 μg/g mouse). For A and B, 30 thrombi in 3 wild-type mice were analyzed.
