Stimulation of TLR2 and TLR4 differentially skews the balance of T cells in a mouse model of arthritis
J. Clin. Invest. Shahla Abdollahi-Roodsaz, et al. 118:205 doi:10.1172/JCI32639 [
Go to this article.]
Figure 5Lower severity and reduced histopathology of
IL1rn–/– arthritis caused by
Tlr4 deficiency.
Similar incidence (
A) and reduced severity (
B) of arthritis in
IL1rn–/–Tlr4–/– as compared with
IL1rn–/–Tlr4+/+ littermates during the first 15 weeks of age. Severity was scored on a scale of 0 to 2 for each paw;
n > 20 mice per group. (
C) Histological assessment of the ankle joints at week 15 of age. Data are mean ± SEM (scale 0–3) of 14 mice per group. mRNA expression of IL-23p19 (
D) and IL-17A (
E) in synovial biopsies of the ankle joints of 15-week-old mice selected according to the degree of inflammation. mRNA expression was measured by quantitative real-time PCR. Relative expression compared with GAPDH (2
-dCt × 10,000) is shown. (
F) Representative images of the ankle joints. Cell influx and chondrocyte death (arrows) were scored on H&E-stained sections (top row), and cartilage and bone damage (arrows) were scored on safranin O–stained tissue sections (bottom row). Original magnification, ×100 for H&E and ×200 for safranin O staining. *
P < 0.05 and ***
P < 0.001.
