Stimulation of TLR2 and TLR4 differentially skews the balance of T cells in a mouse model of arthritis
J. Clin. Invest. Shahla Abdollahi-Roodsaz, et al. 118:205 doi:10.1172/JCI32639 [
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Figure 1Effect of microbial status of
IL1rn–/– mice on the development of arthritis and cytokine response to TCR and TLR stimulations.
The incidence (
A) and severity (
B) of arthritis are completely abolished in germ-free (GF)
IL1rn–/– mice, and infection of GF mice with
Lactobacillus (arrows) leads to the same disease expression as in non–germ-free (NGF) animals. Arthritis severity was scored on a scale from 0 to 2 for each paw;
n ≥ 6 per group. (
C and
D) Splenic lymphocytes (2 × 10
5) were stimulated with plate-coated anti-CD3 (2 μg/ml), Pam3Cys (100 ng/ml), or LPS (200 ng/ml) for 72 hours. IL-1 (
C) and IL-17 (
D) were measured using Luminex multianalyte technology (see Methods). Data are expressed as mean ± SD and are representative of 2 independent experiments.
