JCI - Ablation of GalNAc-4-sulfotransferase-1 enhances reproduction by altering the carbohydrate structures of luteinizing hormone in mice

Ablation of GalNAc-4-sulfotransferase-1 enhances reproduction by altering the carbohydrate structures of luteinizing hormone in mice

Yiling Mi, Dorothy Fiete, Jacques U. Baenziger

Published in Volume 118, Issue 5

J Clin Invest. 2008; 118(5):1815–1824 doi:10.1172/JCI32467

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Figure 9

Clearance of endogenous LH in WT and GalNAc-4-ST1^–/– mice.

WT and GalNAc-4-ST1^–/– 36-week-old mice were surgically castrated or ovariectomized 5 days prior to the clearance studies. Serum was taken from the WT (n = 8) and GalNAc-4-ST1^–/– (n = 8) castrated male mice and from the WT (n = 9) and GalNAc-4-ST1^–/– (n = 6) ovariectomized female mice at the initiation of the clearance study. Acyline (10 μg/mouse) was administered intravenously, and blood was withdrawn at 5, 10, 20, and 30 minutes following introduction of acyline. (A) Comparison of LH clearance by individual castrated WT (squares) and castrated GalNAc-4-ST1^–/– (triangles) mice following acyline injection. Half-lives for a total of 17 WT and 14 GalNAc-4-ST1^–/– mice were determined using GraphPad Prism 4.0. (B) LH removed from the blood of WT mice had a half-life of 7.2 minutes (SEM = 0.6), and LH removed from the blood of GalNAc-4-ST1^–/– mice had a half-life of 10.1 minutes (SEM = 0.9). The difference in half-lives is significant: *P = 0.01.