Ablation of GalNAc-4-sulfotransferase-1 enhances reproduction by altering the carbohydrate structures of luteinizing hormone in mice
J. Clin. Invest. Yiling Mi, et al. 118:1815 doi:10.1172/JCI32467 [Go to this article.]

Figure 2
Generation of GalNAc-4-ST1^–/– mice. (A) A targeting construct containing exons 3 and 4 of mouse GalNAc-4-ST1 flanked by loxP sites followed by PGK-neo also flanked by loxP sites was constructed as illustrated. 5′ and 3′ probes to regions outside of the area used to prepare the targeting construct were used to identify homologous recombinants in ES cells before and after treatment with Cre recombinase. (B) DNA from live progeny of heterozygous matings were genotyped by Southern blotting following EcoRI digestion. The WT product is 11.1 kb and the KO product is 10.3 kb. Examples of WT (+/–) and KO (–/–) progeny are shown.