Glypican-1 modulates the angiogenic and metastatic potential of human and mouse cancer cells
J. Clin. Invest. Takuma Aikawa, et al. 118:89 doi:10.1172/JCI32412 [Go to this article.]

Figure 4
GPC1 expression in tumor xenografts. (A) Immunoblotting. Tumors from sham-transfected and GAS PANC-1 cells (GAS6 and GAS7) were homogenized and incubated with control buffer, heparitinase, or heparitinase and chondroitinase ABC (Ch-ABC). Protein lysates were subjected to immunoblotting using affinity-purified rabbit anti-GPC1 antibody as reported in Methods. Membranes from cell lysates were reprobed with an anti-ERK2 antibody to confirm equivalent loading of lanes. (B) Densitometric analysis. Frozen lysates for immunoblotting from 6 sham, 5 GAS6, and 4 GAS7 tumors were subjected to densitometry. *P < 0.005 compared with sham.