Glypican-1 modulates the angiogenic and metastatic potential of human and mouse cancer cells
J. Clin. Invest. Takuma Aikawa, et al. 118:89 doi:10.1172/JCI32412 [Go to this article.]

Figure 2
Cell growth assays. (A) Effects of GPC1 levels on basal anchorage-independent growth. Sham-transfected PANC-1 cells and GPC1 antisense–expressing clones were seeded in 6-well plates (3,000/well) in complete medium containing 0.3% agar. After 14 days, colonies having more than 20 cells were counted. Data indicate the mean ± SEM of triplicate determinations from 3 experiments. ^#P < 0.01 compared with sham-transfected cells. (B) Effects of GPC1 levels on growth factor–stimulated anchorage-independent growth. Sham-transfected (filled symbols) and GPC1 antisense mRNA–expressing PANC-1 cells (open symbols) were seeded in 6-well plates (3,000/well) in serum-free medium containing 0.3% agar. Serum-free medium containing 1 nM HB-EGF, FGF2, EGF, or IGF-1 was added every 4 days. After 14 days, colonies having more than 20 cells were counted. Data are expressed as percentage change from the respective controls (mean ± SEM) of 3 determinations per experiment from 3 separate experiments. *P < 0.05 and **P < 0.03 compared with control.