Glypican-1 modulates the angiogenic and metastatic potential of human and mouse cancer cells
J. Clin. Invest. Takuma Aikawa, et al. 118:89 doi:10.1172/JCI32412 [Go to this article.]

Figure 1
Effects of GPC1 antisense expression on GPC1 protein levels. (A) Effects of enzymatic treatment on immunoblotting. Sham-transfected cells and GPC1 antisense–expressing cells (clone GAS6) were incubated in the absence or presence of the indicated enzymes and subjected to immunoblotting using affinity-purified rabbit anti-GPC1 antibody as reported in Methods. (B) Total cell lysates from sham-transfected and from both antisense-expressing clones as well as the corresponding conditioned media were incubated with heparitinase and chondroitinase ABC and subjected to immunoblotting as described above. Membranes from cell lysates were reprobed with an anti-ERK2 antibody to confirm equivalent loading of lanes. Each panel is representative of 2 distinct experiments.