Treatment with CD20-specific antibody prevents and reverses autoimmune diabetes in mice
J. Clin. Invest. Chang-yun Hu, et al. 117:3857 doi:10.1172/JCI32405 [Go to this article.]

Figure 8
2H7-treated hCD20 mice have increased CD4 T cells expressing regulatory markers. (A) Splenocytes from hCD20 mice, treated at 9 weeks of age with 2H7 or IgG, were stained with anti-CD4, anti-CD25, and anti-Foxp3 antibodies. A representative flow cytometric plot is illustrated, and percentages shown in the gate represent the Foxp3⁺CD25⁺ cells as a percentage of total CD4 T cells. The graph in the middle is a summary of the values obtained from a number of mice. The graph on the right is a summary of the absolute number of CD4⁺CD25⁺FoxP3⁺ T cells. (B). Splenocytes from hCD20 mice, treated at 9 weeks of age with 2H7 or IgG, were stained with anti-CD4 and CTLA4. The percentages shown in the gate represent the CTLA4⁺ cells as a percentage of total CD4 T cells. The graph in the middle illustrates the values obtained from a number of mice. The graph on the right is a summary of the absolute number of CD4⁺CTLA4⁺ T cells.