Treatment with CD20-specific antibody prevents and reverses autoimmune diabetes in mice
J. Clin. Invest. Chang-yun Hu, et al. 117:3857 doi:10.1172/JCI32405 [
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Figure 7Adoptive transfer of diabetes. (
A) NOD/SCID mice were injected intravenously with (a) 10
7 spleen cells from diabetic mice alone (closed circles); (b) 10
7 spleen cells from diabetic mice cotransferred with spleen cells (10
7) from nondiabetic IgG-treated mice (open circles); (c) 10
7 spleen cells from diabetic mice cotransferred with spleen cells (10
7) from nondiabetic 2H7-treated mice (closed triangles); or (d) spleen cells (10
7) from nondiabetic 2H7-treated mice alone (open squares). There was a significant delay in the onset of diabetes in the group cotransferred with cells from 2H7-treated mice (
P = 0.007). The experiments were performed twice with similar results. Figure
7A shows results of 1 of the 2 experiments. (
B). NOD/SCID mice were injected intravenously with (a) 10
7 spleen cells from diabetic mice alone (filled circles); (b) 10
7 spleen cells from diabetic mice cotransferred with purified CD4 T cells (3 × 10
6) from nondiabetic 2H7-treated mice (open triangles); (c) 10
7 spleen cells from diabetic mice cotransferred with purified splenic B cells (3 × 10
6) from nondiabetic 2H7-treated mice (open circles); (d) purified CD4 T cells (3 × 10
6) alone from nondiabetic 2H7-treated mice (closed squares); and (e) purified splenic B cells alone (3 × 10
6) from nondiabetic 2H7-treated mice (closed triangles). There was a significant delay in the onset of diabetes in the group cotransferred with CD4
+ T cells from 2H7-treated mice compared with spleen cells from diabetic mice alone (
P = 0.029) and in the group cotransferred with purified splenic B cells compared with spleen cells from diabetic mice alone (
P = 0.029). There was no statistical significance between the groups cotransferred with CD4
+ T cells or B cells (
P = 0.28). The results shown in Figure
7B were from 1 of the 2 sets of experiments.
