Distinct KIR/HLA compound genotypes affect the kinetics of human antiviral natural killer cell responses
J. Clin. Invest. Golo Ahlenstiel, et al. 118:1017 doi:10.1172/JCI32400 [
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Figure 4HLA-C–inhibited NK cells of
HLA-C1 homozygous subjects secrete IFN-γ more rapidly after IAV infection than do HLA-C–inhibited NK cells of
HLA-C2 homozygous subjects.
(
A and
C) Kinetic analysis of the IFN-γ response of (
A) HLA-C–inhibited NK cells and (
C) the total NK cell population for a subgroup of
HLA-C1 and
HLA-C2 homozygous subjects. The respective NK cell populations were identified in IAV-infected, CD3-depleted PBMCs in a cytokine-secretion assay by flow cytometry. Mean and SEM at each time point of the infection assay are shown relative to the study end point at 17 h (expressed as ratio).
n = 5 per group. (
B) Frequency of IFN-γ
+, HLA-C–inhibited NK cells at the 9-h time point of the IAV assay relative to the 17-h time point (expressed as 9-h/17-h ratio). (
D) IFN-γ released by the total NK cell population during the first 9h of the IAV assay relative to IFN-γ released during the total 17h of the IAV assay (set as 100%). Experiments in
B and
D were performed as in
A, but included a larger number of
HLA-C1 (
n = 9) and
HLA-C2 homozygous subjects (
n = 8). Horizontal lines indicate the mean.
