Distinct KIR/HLA compound genotypes affect the kinetics of human antiviral natural killer cell responses
J. Clin. Invest. Golo Ahlenstiel, et al. 118:1017 doi:10.1172/JCI32400 [Go to this article.]

Figure 1
Identification and quantitation of HLA-C–inhibited NK cells by flow cytometry. (A) Gating strategy used to identify HLA-C–inhibited NK cells by flow cytometry. After gating on single cells (forward scatter–height versus forward scatter–area) and lymphocytes (forward scatter versus side scatter), NK cells were identified by gating on CD56⁺ cells and by excluding CD3⁺ T cells, CD14⁺ monocytes, CD19⁺ B cells, and EMA⁺ dead cells. Samples from HLA-C1/C1 subjects were stained with antibodies to KIR2DL3, and samples from HLA-C2/C2 subjects were stained with antibodies to KIR2DL1. All samples were stained with antibodies to KIR3DL1 to allow further subset analysis as shown in Figure 5. Numbers indicate the percentage of events in each quadrant. (B) Frequency of HLA-C–inhibited NK cells in PBMCs. The HLA-C–inhibited NK cell subset consists of KIR2DL3⁺ NK cells in HLA-C1 homozygous subjects and KIR2DL1⁺ NK cells in HLA-C2 homozygous subjects. Horizontal lines indicate the mean.