JCI - A shed form of LDL receptor–related protein–1 regulates peripheral nerve injury and neuropathic pain in rodents

A shed form of LDL receptor–related protein–1 regulates peripheral nerve injury and neuropathic pain in rodents

Alban Gaultier, Sanja Arandjelovic, Xiaoqing Li, Julie Janes, Nikola Dragojlovic, George P. Zhou, Jenny Dolkas, Robert R. Myers, Steven L. Gonias, W. Marie Campana

Published in Volume 118, Issue 1

J Clin Invest. 2008; 118(1):161–172 doi:10.1172/JCI32371

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Figure 8

sLRP-α antagonizes TNF-α–initiated cell signaling in astrocytes and microglia.

(A) Astrocyte cultures are immunopositive for GFAP (anti-GFAP, red). Cell nuclei were stained with DAPI (blue). (B) Astrocytes were cultured in serum-free medium for 1 h to reduce the basal level of activation of ERK/MAPK. The cells were then treated with TNF-α (1 nM) or vehicle for 10 min. Cell extracts were subjected to immunoblot analysis to detect P-ERK1/2 and T-ERK1/2. Some cells were pretreated with vehicle or sLRP-α (50 nM) for 10 min prior to adding TNF-α (1.0 nM) for 10 min. The blot is representative of 2 independent studies. (C) Microglial cell cultures were immunopositive for the complement receptor CR3, as determined with antibody OX-42 (green). Cell nuclei were stained with DAPI (blue). (D) Microglia were pretreated with vehicle of sLRP-α for 10 min and then treated with TNF-α (1.0 nM). Extracts were subjected to immunoblot analysis to detect P-p38. β-Actin was used as a loading control.