JCI - Telomerase activity is required for bleomycin-induced pulmonary fibrosis in mice

Telomerase activity is required for bleomycin-induced pulmonary fibrosis in mice

Tianju Liu, Myoung Ja Chung, Matthew Ullenbruch, Hongfeng Yu, Hong Jin, Biao Hu, Yoon Young Choi, Fuyuki Ishikawa, Sem H. Phan

Published in Volume 117, Issue 12

J Clin Invest. 2007; 117(12):3800–3809 doi:10.1172/JCI32369

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Figure 8

Effects of BM transplantation on lung telomerase and fibrosis.

BM chimera mice were generated as described in Methods by transplanting WT BM into TERT^–/– and TERT^–/– BM into WT mice (WT-KO and KO-WT, respectively). BM transplantation from TERT^–/– to TERT^–/– or WT to WT mice were used as sham controls. Whole-lung lysates were collected at day 21 after BLM or saline treatments. (A) Lung telomerase activity was measured by TRAP-based ELISA, and results were expressed as the difference between absorbance at 450 nm and that at 690 nm. (B) Lung collagen content was analyzed with HYP assay. (C) α-SMA expression was detected by Western blotting with equal amounts (5 μg) of protein loaded per lane. A typical blot and the quantitative plot are shown. Data are mean ± SEM. n = 5 (BLM); 3 (saline).