Heparan sulfate and syndecan-1 are essential in maintaining murine and human intestinal epithelial barrier function
J. Clin. Invest. Lars Bode, et al. 118:229 doi:10.1172/JCI32335 [Go to this article.]

Figure 2
Loss of Sdc1 or HS causes increased intestinal protein leakage in mice (A) Sulfated GAG staining with colloidal gold (left and middle columns) and HS staining with bFGF-biotin probe (right column) in small intestinal sections from wild-type (top row), Sdc1^–/– (middle row), and Ext1^Δ/Δ mice (bottom row). The middle column is a magnification of the left column. Original magnification, ×100 (left column); ×400 (middle column); ×200 (right column). (B) HS staining intensity on basolateral surface of IEC in Sdc1^–/– and Ext1^Δ/Δ mice relative to wild-type controls. (C) Intestinal protein leakage (⁵¹Cr or AAT) in Sdc1^–/–, Ext1^Δ/Δ, and HPA-Tg mice relative to respective littermate controls. All data represent assessment in a minimum of n = 5 mice (mean ± SD). **P < 0.01, ***P < 0.001.