Heparan sulfate and syndecan-1 are essential in maintaining murine and human intestinal epithelial barrier function
J. Clin. Invest. Lars Bode, et al. 118:229 doi:10.1172/JCI32335 [Go to this article.]

Figure 1
Sdc1 knockdown in HT29 cells causes protein leakage. (A–E) Sdc1-targeting siRNAs (siRNA1 and siRNA2) reduce Sdc1 mRNA level (A), reduce cell-associated GAGs (B), increase relative albumin leakage (C), reduce TER (D), and amplify TNF-α–induced protein leakage (E), which can be reversed with heparin. Compared with effects of HS loss after exposure to HSase or scrambled siRNA (scr. siRNA) as negative control. White bars represent basal levels without intervention. *P < 0.05, **P < 0.01, ***P < 0.001.