Wnt5a-treated midbrain neural stem cells improve dopamine cell replacement therapy in parkinsonian mice
J. Clin. Invest. Clare L. Parish, et al. 118:149 doi:10.1172/JCI32273 [
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Figure 2Wnt5a mediates effects on DA differentiation via noncanonical signaling across rodent species. (
A and
B) Effects of Wnt5a overexpression were abolished by using a Wnt5a-blocking antibody (W5-ab) or the D4476 CKI inhibitor (CKI), but not the canonical Wnt blocker dickkopf-1 (DKF1). Mouse recombinant Wnt5a protein (r.m.W5) induced effects comparable to those of Wnt5a overexpression in control-transfected spheres. (
C and
D) Comparable trends for number of TH
+ cells per sphere (
C) and percentage of TH
+ spheres per Tuj1/βIII-tubulin
+ spheres (
D) were observed in E12.5 rat compared with E10.5 mouse cultures. However, significantly more TH
+ cells were present in rat cultures than in mouse cultures. Data are mean ± SD (
n = 4). *
P < 0.05; **
P < 0.01; ***
P < 0.001, 1-way ANOVA with Tukey post-hoc test.