Wnt5a-treated midbrain neural stem cells improve dopamine cell replacement therapy in parkinsonian mice
J. Clin. Invest. Clare L. Parish, et al. 118:149 doi:10.1172/JCI32273 [Go to this article.]

Figure 2
Wnt5a mediates effects on DA differentiation via noncanonical signaling across rodent species. (A and B) Effects of Wnt5a overexpression were abolished by using a Wnt5a-blocking antibody (W5-ab) or the D4476 CKI inhibitor (CKI), but not the canonical Wnt blocker dickkopf-1 (DKF1). Mouse recombinant Wnt5a protein (r.m.W5) induced effects comparable to those of Wnt5a overexpression in control-transfected spheres. (C and D) Comparable trends for number of TH⁺ cells per sphere (C) and percentage of TH⁺ spheres per Tuj1/βIII-tubulin⁺ spheres (D) were observed in E12.5 rat compared with E10.5 mouse cultures. However, significantly more TH⁺ cells were present in rat cultures than in mouse cultures. Data are mean ± SD (n = 4). *P < 0.05; **P < 0.01; ***P < 0.001, 1-way ANOVA with Tukey post-hoc test.