(A) Ultrapure LPS augmented antitumor responses in irradiated mice. Mice bearing s.c. B16F10 tumors established for 10 days received 5 Gy TBI. One day later, mice received an ACT treatment consisting of adoptive transfer of 10⁶ cultured pmel-1 T cells, rFPhgp100 vaccination, and rhIL-2 or were left untreated. The next day, mice received ultrapure LPS or were left untreated. Data (mean ± SEM; n = 5–10 per group) are representative of 10 independent experiments. (B) LPS enhanced autoimmune vitiligo in irradiated mice. One month after ACT treatment, irradiated mice treated with or without ultra-pure LPS were evaluated for the development of vitiligo. Mice were scored for the degree of hypopigmentation on a scale of 0–5. Data (n = 4–5 per group) are from 3 independent experiments. Horizontal bars indicate means. (C) Ultrapure LPS increased the absolute number of transferred pmel-1 T cells in the irradiated host. Absolute numbers of transferred pmel-1 T cells (CD8⁺Thy1.1⁺) in irradiated host. Data (mean ± SEM; n = 3–5 per group) are representative of 2 independent experiments. (D) Ultrapure LPS enhanced the function of adoptively transferred cells in irradiated mice. Five days after ACT treatment, pmel-1–Thy1.1⁺ splenocytes were cocultured with irradiated splenocytes pulsed with the indicated doses of hpg100_25–33. Unpulsed splenocytes were used as controls. Data (mean ± SEM; n = 3 per group) are representative of 2 independent experiments. ^††P < 0.05, ^‡‡P < 0.001 versus irradiated treated mice.