Suppression of renal cell carcinoma growth by inhibition of Notch signaling in vitro and in vivo
J. Clin. Invest. Jonas Sjölund, et al. 118:217 doi:10.1172/JCI32086 [Go to this article.]

Figure 3
Inhibition of Notch signaling impairs growth of CCRCC cells. (A) [³H]thymidine incorporation of a panel of CCRCC cells treated for 72 hours with DMSO or DAPT or left untreated (100%). The bars represent mean + SD of 3 independent experiments, each performed 6 times. ***P < 0.001, statistically significant changes (DAPT versus DMSO). (B) 786-O cells treated for 72 hours with DMSO or the alternate γ-secretase inhibitor L-685458 and then analyzed for [³H]-thymidine incorporation. The bars represent mean + SD of 3 independent experiments, each performed 6 times. L-685458–treated cells were normalized to DMSO-treated cells. ***P < 0.001, statistically significant changes (L-685458 versus DMSO). (C) The number of viable (diamonds, DMSO; squares, DAPT) and dead cells (triangles, TB⁺ DMSO; x’s, TB⁺ DAPT) was determined by TB exclusion experiments at indicated times in a panel of CCRCC cells treated with DMSO or DAPT. Results expressed as mean ± SEM of 1 representative experiment performed in triplicate. (D and E) Cell-cycle distribution examined by PI staining and flow cytometry of SKRC-52 cells synchronized by serum starvation and treated with DMSO or DAPT for 24 hours. Results visualized as representative experiment (D) or mean + SD of 3 experiments (E), each performed in triplicate. ***P < 0.001, statistically significant changes (DAPT versus DMSO).