The function of heme-regulated eIF2α kinase in murine iron homeostasis and macrophage maturation
J. Clin. Invest. Sijin Liu, et al. 117:3296 doi:10.1172/JCI32084 [Go to this article.]

Figure 4
Impaired response to LPS treatment in Hri^–/– mice. Serum IL-6 (A), TNF-α (B), and IL-1β (C) levels of Hri^+/+ and Hri^–/– mice at 6 hours after LPS treatment. (D) Hepatic hepcidin level in Hri^+/+ and Hri^–/– mice at 6 hours after LPS treatment. (E) Hepatic, splenic, and serum iron content in Hri^+/+ and Hri^–/– mice at 24 hours after LPS treatment. Hepatic iron content in Hri^+/+ and Hri^–/– mice was not significantly changed after LPS treatment. Splenic iron content was significantly increased after LPS treatment in both Hri^+/+ and Hri^–/– mice (P < 0.05); however, the splenic iron content after LPS treatment in Hri^–/– mice was significantly lower than that in Hri^+/+ mice (P < 0.01). Serum iron was significantly decreased after LPS treatment in both Hri^+/+ and Hri^–/– mice (P < 0.001); however, the serum iron in Hri^–/– mice was significantly higher than that in Hri^+/+ mice after LPS treatment (P < 0.01). Four-month-old male mice were used, and hepcidin mRNA was analyzed as described in the legend of Figure 1. Hepcidin expression in Hri^+/+ was defined as 1. Results are presented as mean ± SEM (n = 6–9).