The function of heme-regulated eIF2α kinase in murine iron homeostasis and macrophage maturation
J. Clin. Invest. Sijin Liu, et al. 117:3296 doi:10.1172/JCI32084 [
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Figure 3Impairment of macrophage maturation and LPS response in
Hri–/– BMDMs.
(
A) Cell morphology of
Hri+/+ and
Hri–/– BMDM cultures. Phase-contrast images of BMDMs at days 3 and 6; original magnification, ×200. (
B) FACS analyses of expression of macrophage surface markers F4/80 and CD11b. The
y axes shows peak intensity (AU). (
C) Western blot analyses of CSF-1R protein expression at days 3 and 6. The ratios of CSF-1R to eIF2α are shown above the autoradiogram. (
D) FACS analyses of TLR4 protein expression at day 6. The peak intensity is shown in the upper-right corner of each diagram (
P < 0.01). (
E) Production of TNF-α and IL-6 upon LPS treatment. TNF-α and IL-6 in the culture media were measured by ELISA. Bone marrow macrophages from 6- to 8-week-old male mice were cultured, and results are presented as mean ± SEM (
n = 3–4).
