The function of heme-regulated eIF2α kinase in murine iron homeostasis and macrophage maturation
J. Clin. Invest. Sijin Liu, et al. 117:3296 doi:10.1172/JCI32084 [Go to this article.]

Figure 2
Expression of HRI protein in BMDMs. (A) Immuno­fluorescence staining of HRI and Mac-1 in Hri^+/+ and Hri^–/– macrophages. Original magnification, ×400. (B) Western blot analyses of HRI protein, eIF2αP, and total eIF2α levels in Hri^+/+ and Hri^–/– macrophages and E14.5 fetal liver cells. The intensities of autoradiograms were quantitated as described in Methods. The ratios of eIF2αP to eIF2α were calculated; the ratio in Hri^+/+ was defined as 1. The normalized ratios are shown above the autoradiogram.