The function of heme-regulated eIF2α kinase in murine iron homeostasis and macrophage maturation
J. Clin. Invest. Sijin Liu, et al. 117:3296 doi:10.1172/JCI32084 [
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Figure 1Effects of HRI and iron deficiencies on tissue iron, serum iron, and hepatic hepcidin expression. (
A) Hepatic, splenic, and serum iron content in
Hri+/+ and
Hri–/– mice. (
B) Hepatic, splenic, and serum iron contents in iron-deficient
Hri+/+ and
Hri–/– mice. (
C) Hepatic hepcidin mRNA in
Hri+/+ and
Hri–/– mice during iron deficiency. Hepcidin expression was significantly decreased in iron deficiency (
P < 0.01, compared with respective control mice). Hepcidin expression was also significantly decreased in
Hri–/– mice (
P < 0.05 for both iron-sufficient and iron-deficient conditions). Serum and tissue samples were collected from 4-month-old mice. Hepcidin mRNA was analyzed by qRT-PCR and normalized with eIF2α. Hepcidin expression in
Hri+/+ was defined as 1. Results are presented as mean ± SEM (
n = 6–9).
