Myocardial hypertrophy in the absence of external stimuli is induced by angiogenesis in mice
J. Clin. Invest. Daniela Tirziu, et al. 117:3188 doi:10.1172/JCI32024 [Go to this article.]

Figure 4
In vitro effects of PR39 administration. (A) Surface area of rat neonatal cardiomyocytes transduced with Ad-PR39 (100 MOI) or treated with PR39 (1 μM) or Ang II (1 μM) peptides for 72 hours (n = 150 cells each). (B) RT-PCR analysis of hypertrophic markers ANF, β-MHC, and skeletal α-actin after 72 hours of exposure to PR39 (adenoviral vector or peptide) or Ang II. (C) Stimulation of protein synthesis assessed by ³H-leucine incorporation (n = 3 independent experiments). (D) Micrographs of rat neonatal cardiomyocytes transduced with Ad-PR39 (100 MOI) or treated with PR39 peptide (1 μM) or Ang II (1 μM) for 72 hours, immunostained with α-actinin sarcomeric Ab. **P < 0.01. Scale bars: 50 μm.