Myocardial hypertrophy in the absence of external stimuli is induced by angiogenesis in mice
J. Clin. Invest. Daniela Tirziu, et al. 117:3188 doi:10.1172/JCI32024 [Go to this article.]

Figure 3
Myocardial hypertrophy in tTA⁺/PR39⁺ mice. (A) Isolated cardiomyocytes from tTA⁺/PR39⁺ mice after 6 weeks induction compared with control. Scale bar: 100 μm. (B) Quantification of cross-sectional area of isolated cardiomyocytes. 50 cells/ group; *P < 0.01. (C) RT-PCR analysis of hypertrophic markers (ANF, β-MHC, skeletal α-actin) after PR39 induction for 3 weeks, 6 weeks, and 6ON/3OFF. GAPDH expression was used as a control. Note the high level of expression of hypertrophic markers for 6ON/3OFF compared with control (D). Western blot of Akt activation (phospho–Akt-Ser473), Erk1/2 (phospho-p44/42 MAPK Tyr202/Thr204), and transcription factor GATA4 after PR39 induction for 3 weeks, 6 weeks, and 6ON/3OFF.