Myocardial hypertrophy in the absence of external stimuli is induced by angiogenesis in mice
J. Clin. Invest. Daniela Tirziu, et al. 117:3188 doi:10.1172/JCI32024 [Go to this article.]

Figure 2
Histological analysis of tTA⁺/PR39⁺ hearts after 6-weeks induction. (A) Interstitial fibrosis as assessed by Masson’s trichrome staining (MT). Note the absence of fibrosis in tTA⁺/PR39⁺ mice induced for 6 weeks. (B and C) Apoptosis assessed by double DAPI and TUNEL staining (B) and its quantification (C). (D) Cell proliferation assessed with anti-Ki67 Ab and DAPI and lectin staining. Note association of Ki67 antigen expression with endothelial cells (arrows). (E) Quantification of proliferation. Note an increase of cell proliferation in tTA⁺/PR39⁺ mice compared with control mice. *P < 0.001 (n = 5 mice/group). Scale bars: 50 μm.