Osteopontin mediates obesity-induced adipose tissue macrophage infiltration and insulin resistance in mice
J. Clin. Invest. Takashi Nomiyama, et al. 117:2877 doi:10.1172/JCI31986 [
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Figure 4OPN deficiency decreases ATM content in obese mice. (
A) ATM content was determined by immunohistochemical analysis of epididymal adipose tissues isolated from
OPN+/+ and
OPN–/– mice fed a LFD or HFD. Adipose tissues were stained using an absorbed rabbit anti-mouse macrophage antiserum (original magnification, ×100). (
B) Epididymal adipose tissues from obese
OPN+/+ and
OPN–/– mice were analyzed for macrophage content using an F4/80 antibody (magnified as indicated). (
C) Macrophage content was quantified by analyzing the fraction of F4/80-stained cells relative to total number of cells in epididymal adipose tissue from
OPN+/+ (black bars) and
OPN–/– (white bars) mice fed a LFD or HFD (
n = 8/group). Values are expressed as mean ± SEM. (
D) Macrophage content was quantitatively assessed by real-time RT-PCR for CD68 mRNA expression in EWAT, the AF, and the SVF isolated from
OPN+/+ (black bars) and
OPN–/– (white bars) mice (
n = 12/group) fed a LFD or HFD for 25 weeks. Data are presented as relative CD68 mRNA expression normalized to TFIIB mRNA expression and are expressed as mean ± SEM. *
P < 0.05, HFD compared with LFD;
#P < 0.05,
OPN–/– mice compared with
OPN+/+ mice fed HFD.
