Osteopontin mediates obesity-induced adipose tissue macrophage infiltration and insulin resistance in mice
J. Clin. Invest. Takashi Nomiyama, et al. 117:2877 doi:10.1172/JCI31986 [
Go to this article.]
Figure 1OPN plasma levels in DIO and expression of OPN in adipose tissue. C57BL/6 mice (
n = 10/group) were fed a LFD or HFD for 20 weeks. (
A) OPN plasma levels were analyzed by ELISA and data expressed as mean ± SEM.
#P < 0.005. (
B) Adipose tissues were isolated and OPN mRNA expression was analyzed in whole EWAT, the AF, and the SVF. Data are presented as relative OPN mRNA expression normalized to TFIIB mRNA expression and are expressed as mean ± SEM. *
P < 0.05, compared with LFD. (
C) SVFs isolated from EWATs of obese wild-type mice (
n = 6) were pooled and separated into macrophages, endothelial cells, and preadipocytes using magnetic immunoaffinity isolation. Cell fractions were analyzed for OPN (black bars, left
y axis) and CD68 (white bars, right
y axis) mRNA expression. Data are presented as mRNA expression relative to TFIIB mRNA expression and are expressed as mean ± SEM. **
P < 0.01 compared with preadipocyte or endothelial cell fraction. (
D) Paraffin-embedded EWAT was analyzed for OPN immunoreactivity and F4/80-positive macrophage content. Sections were counterstained with hematoxylin and images obtained at the indicated magnifications.
