Adipocyte LDL receptor–related protein–1 expression modulates postprandial lipid transport and glucose homeostasis in mice
J. Clin. Invest. Susanna M. Hofmann, et al. 117:3271 doi:10.1172/JCI31929 [
Go to this article.]
Figure 1Characteristics of 2 independently generated
ad-Lrp1+/+ and
ad-Lrp1–/– mouse colonies.
Adipose tissue–specific LRP1 gene inactivation was performed both on a mixed background (Dallas) and on an inbred C57BL/6 background (Cincinnati) by crossing aP2
Cre transgenic mice with
Lrp1flox/flox mice. (
A) Detection of the Cre transgene in mice with the mixed background by PCR. A fragment of 400 bp was amplified from mouse tail genomic DNA when the
Cre transgene driven by the aP2 promoter was present. A 200-bp fragment amplified from the
aP2 gene was observed in both PCR products as control. (
B) Detection of wild-type (+/+) and mutant (–/–) mRNA of mice (mixed background) by quantitative real-time PCR. (
C) Western blot identification of the β subunit of LRP1 in epididymal WAT (eWAT), BAT, and brain of
ad-Lrp1+/+ and
ad-Lrp1–/– mice (mixed background). β-Actin was used as loading control. (
D) Ponceau S staining and Western blot of the β subunit of LRP1 in the stromal-vascular cells (sv) and mature adipocytes of subcutaneous (sWAT) and eWAT adipose tissue in
ad-Lrp1+/+ and
ad-Lrp1–/– mice. (
E) Western blot of the β subunit of LRP1 in BAT (lanes 1 and 2), epididymal WAT (eWAT) (lanes 3 and 4), liver (lanes 5 and 6), skeletal muscle (lanes 7 and 8), and peritoneal macrophages (lanes 9, 10) of
ad-Lrp1+/+ (lanes 1, 3, 5, 7, and 9) and
ad-Lrp1–/– (lanes 2, 4, 6, 8, and 10) mice (inbred background). Molecular size markers (Mr) were applied to the lane flanking the sample lanes as indicated.
