TNF-α induces leukemic clonal evolution ex vivo in Fanconi anemia group C murine stem cells
J. Clin. Invest. June Li, et al. 117:3283 doi:10.1172/JCI31772 [
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Figure 4TNF-α induces overprotection of ROS in
Fancc–/– leukemic mice.
ROS production in transplanted recipient mice. 1 × 10
6 TNF-α–treated WT cells or
Fancc–/– preleukemic cells were injected i.v. into lethally irradiated recipients, which after 10 days were injected i.p. with 1 dose (100 μg/kg) of TNF-α followed by NAC (100 mg/kg/d; TNF + NAC) or PBS (TNF + PBS) for 10 days. When recipients of
Fancc–/– preleukemic cells became moribund, 12 (6 each in TNF + NAC or TNF + PBS) were analyzed in parallel with 12 recipients of WT cells (6 each in TNF + NAC or TNF + PBS). BM cells were stained with fluorescent probe CM-H
2DCFDA, and ROS were detected by flow cytometry. Shown is a representative flow cytometric analysis of ROS production in BM cells from the indicated recipient mice.
